【英文标准名称】:Foodstuffs-Methodsofanalysisforthedetectionofgeneticallymodifiedorganismsandderivedproducts-Qualitativenucleicacidbasedmethods
【原文标准名称】:食品.转基因有机体和衍生物探测分析法.定性核酸法
【标准号】:BSENISO21569-2005
【标准状态】:现行
【国别】:英国
【发布日期】:2005-10-21
【实施或试行日期】:2005-10-21
【发布单位】:英国标准学会(GB-BSI)
【起草单位】:BSI
【标准类型】:()
【标准水平】:()
【中文主题词】:分析;食物链反应;化学分析和试验;污染;定义;DNA;食品检验;食品;食品检验;基因改进;基因产品;遗传工程;在实验室条件下;说明书;调查;实验室;实验室设备;实验室试验;分析方法;微生物食品;微生物分析;微生物学;微生物;最低要求;核酸;生物体;性能要求;聚合酶;聚合酶食物链;质量保证;筛分法;规范(验收);试验;验证
【英文主题词】:Analysis;Chainreactions;Chemicalanalysisandtesting;Contamination;Definition;Definitions;DNA;Foodinspection;Foodproducts;Foodtesting;Genemodified;Geneproduct;Geneticengineering;Invitro;Instructions;Investigations;Laboratories;Laboratoryequipment;Laboratorytesting;Laboratorytests;Methodsofanalysis;Microbialfoods;Microbiologicalanalysis;Microbiology;Microorganisms;Minimumrequirements;Nucleicacids;Organisms;PCR;Performancerequirements;Polymerase;Polymerasechainreaction;Qualityassurance;Screening-method;Specification(approval);Testing;Verification
【摘要】:ThisInternationalStandarddescribestheproceduretoqualitativelydetectgeneticallymodifiedorganisms(GMOs)andderivedproductsbyanalysingthenucleicacidsextractedfromthesampleunderstudy.Themainfocusisonpolymerasechainreaction(PCR)basedamplificationmethods.Itgivesgeneralrequirementsforthespecificdetectionandidentificationoftargetnucleicacidsequences(DNA)andfortheconfirmationoftheidentityoftheamplifiedDNAsequence.Guidelines,minimumrequirementsandperformancecriterialaiddowninthisInternationalStandardareintendedtoensurethatcomparable,accurateandreproducibleresultsareobtainedindifferentlaboratories.ThisInternationalStandardhasbeenestablishedforfoodmatrices,butcouldalsobeappliedtoothermatrices(e.g.feedandplantsamplesfromtheenvironment).SpecificexamplesofmethodsareprovidedinAnnexesAtoD.
【中国标准分类号】:C53
【国际标准分类号】:67_050
【页数】:80P.;A4
【正文语种】:英语